Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

mRNA export from the nucleus requires the RNA helicase UAP56 and involves remodeling of ribonucleo-protein complexes in the nucleus. Here, we show that UAP56 is required for bulk mRNA export from the nurse cell nuclei that supply most of the material to the growing Drosophila oocyte and for the organization of chromatin in the oocyte nucleus. Loss of UAP56 function leads to patterning defects that identify uap56 as a spindle-class gene similar to the RNA helicase Vasa. UAP56 is required for the localization of gurken, bicoid and oskar mRNA as well as post-translational modification of Osk protein. By injecting grk RNA into the oocyte cytoplasm, we show that UAP56 plays a role in cytoplasmic mRNA localization. We propose that UAP56 has two independent functions in the remodeling of ribonucleo-protein complexes. The first is in the nucleus for mRNA export of most transcripts from the nucleus. The second is in the cytoplasm for remodeling the transacting factors that decorate mRNA and dictate its cytoplasmic destination.

Original publication

DOI

10.1016/j.ydbio.2007.12.004

Type

Journal article

Journal

Dev Biol

Publication Date

01/03/2008

Volume

315

Pages

89 - 98

Keywords

Active Transport, Cell Nucleus, Animals, Body Patterning, Cell Nucleus, Cytoplasm, Drosophila, Female, Immunohistochemistry, In Situ Hybridization, Microinjections, Oocytes, Oogenesis, Protein Processing, Post-Translational, RNA Helicases, RNA, Messenger