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© 2019 Optical Society of America. We present IsoSense, a wavefront sensing method that mitigates sample dependency in image-based sensorless adaptive optics applications in microscopy. Our method employs structured illumination to create additional high spatial frequencies in the image through custom illumination patterns. This improves the reliability of image quality metric calculations and enables sensorless wavefront measurement even in samples with sparse spatial frequency content. We demonstrate the feasibility of IsoSense for aberration correction in a deformable-mirror-based structured illumination super-resolution fluorescence microscope. © 2019 Optical Society of America under the terms of the OSA Open Access.

Original publication

DOI

10.1364/OPTICA.6.000370

Type

Journal article

Journal

Optica

Publication Date

20/03/2019

Volume

6

Pages

370 - 379