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The 'silent' yeast mating-type loci (HML and HMR) are repressed by sequences (HMLE and HMRE) located over 1 kb from their promoters which have properties opposite those of enhancers, and are called 'silencers'. Both silencers contain autonomously replicating sequences (ARS). Silencer activity requires four trans-acting genes called SIR (silent information regulator). We have identified two DNA binding factors , SBF-B and SBF-E, which bind to known regulatory elements at HMRE. SBF-B binds to a region involved in both the silencer and ARS functions of HMRE, but doesn not bind to HMLE. This factor also binds to the unlinked ARS1 element. SBF-E recognizes a sequence found at both silencers. These results suggest that the two silencers may be composed of different combinations of regulatory elements at least one of which is common to both. Neither factor appears to be a SIR gene product. Hence the SIR proteins may not directly interact with the silencer control sites.

Type

Journal article

Journal

EMBO J

Publication Date

02/1987

Volume

6

Pages

461 - 467

Keywords

DNA Replication, Genes, Fungal, Genes, Mating Type, Fungal, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Silencer Elements, Transcriptional, Silent Information Regulator Proteins, Saccharomyces cerevisiae