Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Halving of the chromosome number during meiosis I depends on the segregation of maternal and paternal centromeres. This process relies on the attachment of sister centromeres to microtubules emanating from the same spindle pole. We describe here the identification of a protein complex, Csm1/Lrs4, that is essential for monoorientation of sister kinetochores in Saccharomyces cerevisiae. Both proteins are present in vegetative cells, where they reside in the nucleolus. Only shortly before meiosis I do they leave the nucleolus and form a "monopolin" complex with the meiosis-specific Mam1 protein, which binds to kinetochores. Surprisingly, Csm1's homolog in Schizosaccharomyces pombe, Pcs1, is essential for accurate chromosome segregation during mitosis and meiosis II. Csm1 and Pcs1 might clamp together microtubule binding sites on the same (Pcs1) or sister (Csm1) kinetochores.

Type

Journal article

Journal

Dev Cell

Publication Date

04/2003

Volume

4

Pages

535 - 548

Keywords

ATP-Binding Cassette Transporters, Cell Cycle Proteins, Cell Nucleolus, Centromere, Chromosomal Proteins, Non-Histone, Chromosome Segregation, DNA, Gene Expression Regulation, Fungal, Kinetochores, Macromolecular Substances, Meiosis, Molecular Sequence Data, Mutation, Nuclear Proteins, Phosphoproteins, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid