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Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release (FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEARnetwork promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.

Original publication

DOI

10.1126/science.1099402

Type

Journal article

Journal

Science

Publication Date

23/07/2004

Volume

305

Pages

516 - 519

Keywords

Anaphase, Cell Cycle Proteins, Cell Nucleolus, Cyclin B, Cyclin B1, Cyclin-Dependent Kinases, DNA, Ribosomal, Meiosis, Metaphase, Mitosis, Mutation, Nuclear Proteins, Phosphorylation, Protein Kinases, Protein Tyrosine Phosphatases, Protein-Serine-Threonine Kinases, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins