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The intracellular localization of the S. cerevisiae transcription factor SWI5 is cell cycle dependent. The protein is nuclear in G1 cells but cytoplasmic in S, G2, and M phase cells. We have identified SWI5's nuclear localization signal (NLS) and show that it can confer cell cycle-dependent nuclear entry to a heterologous protein. Located within or close to the NLS are three serine residues, mutation of which results in constitutive nuclear entry. These residues are phosphorylated in a cell cycle-dependent manner in vivo, being phosphorylated when SWI5 is in the cytoplasm and dephosphorylated when it is in the nucleus. As all three serines are phosphorylated by purified CDC28-dependent H1 kinase activity in vitro, we propose a model in which the CDC28 kinase acts directly to control nuclear entry of SWI5.

Type

Journal article

Journal

Cell

Publication Date

23/08/1991

Volume

66

Pages

743 - 758

Keywords

Amino Acid Sequence, Cell Compartmentation, Cell Cycle, Cell Cycle Proteins, Cell Nucleus, Cloning, Molecular, DNA Mutational Analysis, DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Histones, Maturation-Promoting Factor, Molecular Sequence Data, Peptide Mapping, Phosphoproteins, Phosphorylation, Protamine Kinase, Protein Kinases, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic