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Pheromone-stimulated haploid yeast cells undergo a differentiation process that allows them to mate. Transmission of the intracellular signal involves threonine and tyrosine phosphorylation of the redundant FUS3 and KSS1 kinases, which are members of the MAP kinase family. FUS3/KSS1 phosphorylation depends on two additional kinases, STE11 and STE7 (refs 2, 5, 6). Genetic analyses predict an ordered pathway where STE11 acts before STE7 and FUS3/KSS1 (refs 2, 7). Here we report that STE7 is a dual-specificity kinase that modifies FUS3 at the appropriate sites and stimulates its catalytic activity in vitro. From these data and previous genetic results, we argue that STE7 is the physiological activator of FUS3. Recent indications that MAP kinase activators are related to STE7 suggest that signal transduction pathways in many, if not all, eukaryotic organisms use homologous kinase cascades.

Original publication

DOI

10.1038/362261a0

Type

Journal article

Journal

Nature

Publication Date

18/03/1993

Volume

362

Pages

261 - 264

Keywords

Calcium-Calmodulin-Dependent Protein Kinases, Electrophoresis, Polyacrylamide Gel, Enzyme Activation, Escherichia coli, Fungal Proteins, Mitogen-Activated Protein Kinase Kinases, Mitogen-Activated Protein Kinases, Molecular Weight, Phosphorylation, Protein Kinases, Protein-Tyrosine Kinases, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins