Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Homothallic yeast cells undergo a specific pattern of mating-type switching initiated by an endonuclease encoded by the HO gene. HO transcription is affected by cell type (a, alpha, and a/alpha), by cell age (mother or daughter), and by the cell cycle. This paper investigates the sequences involved in HO transcription by replacing genomic DNA with copies mutated in vitro. A region between -1000 and 1400 (called URS1) is necessary for transcription in addition to a "TATA"-like region at -90. The 900 bp of DNA separating URS1 from the "TATA" box is not necessary for transcription nor for a/alpha repression and some measure of mother/daughter control, but it is necessary for correct cell cycle control.

Type

Journal article

Journal

Cell

Publication Date

08/1985

Volume

42

Pages

213 - 223

Keywords

Base Sequence, Cell Cycle, Chromosome Deletion, DNA, Fungal, Endodeoxyribonucleases, Gene Expression Regulation, Genes, Fungal, Genes, Mating Type, Fungal, Promoter Regions, Genetic, RNA, Fungal, Saccharomyces cerevisiae, Transcription, Genetic