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Cohesion between sister chromatids depends on a multisubunit cohesin complex that binds to chromosomes around DNA replication and dissociates from them at the onset of anaphase. Scc2p, though not a cohesin subunit, is also required for sister chromatid cohesion. We show here that Scc2p forms a complex with a novel protein, Scc4p, which is also necessary for sister cohesion. In scc2 or scc4 mutants, cohesin complexes form normally but fail to bind both to centromeres and to chromosome arms. Our data suggest that a major role for the Scc2p/Scc4p complex is to facilitate the loading of cohesin complexes onto chromosomes.

Type

Journal article

Journal

Mol Cell

Publication Date

02/2000

Volume

5

Pages

243 - 254

Keywords

Cell Cycle Proteins, Chromatids, Chromatin, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Chromosomes, Fungal, Fungal Proteins, Nuclear Proteins, Protein Binding, Saccharomyces cerevisiae Proteins, Yeasts