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The interconversion of mating types in yeast is initiated by an endonuclease encoded by the HO gene. HO is transcribed only transiently during the G1 phase of the cell cycle, as cells undergo START. A deletion analysis of HO 5'-flanking DNA suggests that there must be multiple copies of the sequence that confers START-specific transcription in the interval from -150 to -900. Analysis of this interval revealed 10 occurrences of sequences that closely match the consensus Pur N N Pyr C A C G A4. To test whether these sequences are the putative cell-cycle control elements, synthetically derived copies of the consensus were inserted at the break points of constitutive deletions and shown to restore START-dependent cell-cycle control.

Type

Journal article

Journal

Cell

Publication Date

08/1985

Volume

42

Pages

225 - 235

Keywords

Cell Cycle, Chromosome Deletion, DNA, Fungal, DNA, Recombinant, Endodeoxyribonucleases, Genes, Fungal, Interphase, Mutation, Promoter Regions, Genetic, Repetitive Sequences, Nucleic Acid, Saccharomyces cerevisiae, Transcription, Genetic