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Two rounds of chromosome segregation after only a single round of DNA replication enable the production of haploid gametes from diploid precursors during meiosis. To identify genes involved in meiotic chromosome segregation, we developed an efficient strategy to knock out genes in the fission yeast on a large scale. We used this technique to delete 180 functionally uncharacterized genes whose expression is upregulated during meiosis. Deletion of two genes, sgo1 and mde2, caused massive chromosome missegregation. sgo1 is required for retention of centromeric sister-chromatid cohesion after anaphase I. We show here that mde2 is required for formation of the double-strand breaks necessary for meiotic recombination.

Original publication

DOI

10.1016/j.cub.2005.07.059

Type

Journal article

Journal

Curr Biol

Publication Date

20/09/2005

Volume

15

Pages

1663 - 1669

Keywords

Chromatids, Chromosomal Proteins, Non-Histone, Chromosome Segregation, DNA Primers, Forkhead Transcription Factors, Gene Deletion, Gene Expression Profiling, Genetic Vectors, Green Fluorescent Proteins, Hygromycin B, Meiosis, Microscopy, Fluorescence, Polymerase Chain Reaction, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Streptothricins