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Parkinson's disease is the second most common neurodegenerative disease without cure. It is characterized by α-synuclein accumulation and aggregation in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in Drosophila has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phenotype, quantification of dopaminergic neuronal loss, and measurements of circadian effects.

Original publication




Journal article


Methods Mol Biol

Publication Date





199 - 208


Behavior, Drosophila, In vivo models, Morphology analysis, Neurotoxicity, Animals, Animals, Genetically Modified, Behavior, Animal, Biological Assay, Biomarkers, Circadian Rhythm, Disease Models, Animal, Dopaminergic Neurons, Drosophila, Locomotion, Parkinson Disease, alpha-Synuclein