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Parkinson's disease is the second most common neurodegenerative disease without cure. It is characterized by α-synuclein accumulation and aggregation in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in Drosophila has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phenotype, quantification of dopaminergic neuronal loss, and measurements of circadian effects.

Original publication

DOI

10.1007/978-1-4939-9124-2_15

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2019

Volume

1948

Pages

199 - 208

Keywords

Behavior, Drosophila, In vivo models, Morphology analysis, Neurotoxicity, Animals, Animals, Genetically Modified, Behavior, Animal, Biological Assay, Biomarkers, Circadian Rhythm, Disease Models, Animal, Dopaminergic Neurons, Drosophila, Locomotion, Parkinson Disease, alpha-Synuclein