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Deletion analysis was carried out on the human 7SK RNA gene to map regions essential for in vitro transcription by RNA polymerase III. The sequence promoting transcription is located between 37 and 3 bp upstream of the 7SK RNA coding region. RNA polymerase III transcription of adjacent plasmid sequences can be directed by this promoter in the complete absence of the 7SK RNA coding region, indicating that no internal promoter sequences are required. Transcription is terminated by a stretch of T residues, typical of RNA polymerase III transcription. The promoter contains a TATA box at position -25, mutations within which dramatically reduce the efficiency of transcription. Upstream sequences from position -37 to -243 increase the promoter's efficiency. The promoter recognized by RNA polymerase III is structurally and functionally similar to the promoter of genes transcribed by RNA polymerase II.

Type

Journal article

Journal

Cell

Publication Date

09/10/1987

Volume

51

Pages

81 - 87

Keywords

Amanitins, Base Sequence, DNA, Recombinant, DNA-Directed RNA Polymerases, Electrophoresis, Polyacrylamide Gel, Humans, Molecular Sequence Data, Mutation, Plasmids, Promoter Regions, Genetic, RNA Polymerase III, RNA, Small Nuclear, Regulatory Sequences, Nucleic Acid, Transcription, Genetic