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The guanosine triphosphatases (GTPases) of the immunity-associated protein (GIMAP) family of putative GTPases has been implicated in the regulation of T-lymphocyte development and survival. A mouse conditional knockout allele was generated for the immune GTPase gene GIMAP1. Homozygous loss of this allele under the influence of the lymphoid-expressed hCD2-iCre recombinase transgene led to severe (> 85%) deficiency of mature T lymphocytes and, unexpectedly, of mature B lymphocytes. By contrast there was little effect of GIMAP1 deletion on immature lymphocytes in either B or T lineages, although in vitro studies showed a shortening of the survival time of both immature and mature CD4(+) single-positive thymocytes. These findings show a vital requirement for GIMAP1 in mature lymphocyte development/survival and draw attention to the nonredundant roles of members of the GIMAP GTPase family in these processes.

Original publication

DOI

10.1182/blood-2009-08-237586

Type

Journal article

Journal

Blood

Publication Date

22/04/2010

Volume

115

Pages

3249 - 3257

Keywords

Animals, B-Lymphocytes, Blotting, Western, Cell Differentiation, Cell Separation, Cell Survival, Flow Cytometry, GTP Phosphohydrolases, Mice, Mice, Knockout, Polymerase Chain Reaction, Signal Transduction, T-Lymphocytes