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Vav, a guanine nucleotide exchange factor for members of the Rho family of small GTPases, is activated through engagement of B and T lymphocyte antigen receptors. It is important for establishing the signaling threshold of the TCR, as mice lacking Vav display defective thymocyte selection. Here, conventional B cells are shown to develop normally in Vav-deficient mice but these mice have few B-1 B cells. The threshold for inducing B cell proliferation through BCR engagement in vitro is greater in Vav-deficient B cells. Nevertheless, in vivo the mutant mice have normal antibody responses to haptenated Ficoll. In contrast, Vav-/- mice show defective class switching to IgG and germinal center formation when immunized with haptenated protein. Interestingly, this defect is reversed in chimeras where normal T cells are present. Antigen-specific proliferation of T cells in the T zone was found to be similar in wild-type and Vav-/- mice but the induction of IL-4 mRNA and switch transcripts was specifically impaired. These results suggest that defective immunoglobulin class switching in Vav-deficient mice is attributable to compromised T cell help.

Original publication

DOI

10.1002/(SICI)1521-4141(199902)29:02<477::AID-IMMU477>3.0.CO;2-V

Type

Journal article

Journal

Eur J Immunol

Publication Date

02/1999

Volume

29

Pages

477 - 487

Keywords

Animals, B-Lymphocytes, Cell Cycle Proteins, Gene Expression Regulation, Immunoglobulin Class Switching, Lymphocyte Activation, Lymphocyte Cooperation, Mice, Mice, Knockout, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-vav, T-Lymphocytes