Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We found upregulation of expression of the microRNA miR-155 in primary effector and effector memory CD8(+) T cells, but low miR-155 expression in naive and central memory cells. Antiviral CD8(+) T cell responses and viral clearance were impaired in miR-155-deficient mice, and this defect was intrinsic to CD8(+) T cells, as miR-155-deficient CD8(+) T cells mounted greatly diminished primary and memory responses. Conversely, miR-155 overexpression augmented antiviral CD8(+) T cell responses in vivo. Gene-expression profiling showed that miR-155-deficient CD8(+) T cells had enhanced type I interferon signaling and were more susceptible to interferon's antiproliferative effect. Inhibition of the type I interferon-associated transcription factors STAT1 or IRF7 resulted in enhanced responses of miR-155-deficient CD8(+) T cells in vivo. We have thus identified a previously unknown role for miR-155 in regulating responsiveness to interferon and CD8(+) T cell responses to pathogens in vivo.

Original publication

DOI

10.1038/ni.2576

Type

Journal article

Journal

Nat Immunol

Publication Date

06/2013

Volume

14

Pages

593 - 602

Keywords

Animals, CD8-Positive T-Lymphocytes, Cell Proliferation, Flow Cytometry, Gene Expression Profiling, Gene Regulatory Networks, Immunoblotting, Immunologic Memory, Interferon Regulatory Factor-7, Interferons, Mice, Mice, Inbred C57BL, Mice, Knockout, MicroRNAs, Oligonucleotide Array Sequence Analysis, Orthomyxoviridae, Orthomyxoviridae Infections, RNA Interference, Reverse Transcriptase Polymerase Chain Reaction, STAT1 Transcription Factor, Signal Transduction, Suppressor of Cytokine Signaling 1 Protein, Suppressor of Cytokine Signaling Proteins