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B cells from phospholipase C (PLC)gamma2-deficient mice express reduced levels of the pro-survival protein Bcl-2 and show a defect in the development of transitional T3 and marginal zone (MZ) B cells that reflects reduced B cell survival. Introduction of a bcl-2 transgene restored the numbers of MZ, T3 and follicular B cells in PLCgamma2(-/-) mice. Restricting the B cell repertoire in PLCgamma2-deficient mice by the introduction of a BCR transgene resulted in a striking reduction in the number of IgM-positive B cells and a paucity of IgD-expressing cells in the spleen which was also rescued by the bcl-2 transgene. BCR-stimulated ERK and IkappaBalpha phosphorylation were PLCgamma2 dependent, while calcium flux was reduced, but not abrogated, in the absence of PLCgamma2, suggesting an ancillary role for PLCgamma1. The bcl-2 transgene rescued development of PLCgamma2(-/-) B cells and serum IgM levels but did not restore BCR-mediated signaling, proliferation or serum IgG3 levels. These data suggest that PLCgamma2 performs a critical role in B cell development through regulation of survival rather than differentiation.

Original publication

DOI

10.1002/eji.200425054

Type

Journal article

Journal

Eur J Immunol

Publication Date

08/2004

Volume

34

Pages

2237 - 2247

Keywords

Animals, B-Lymphocyte Subsets, B-Lymphocytes, Cell Differentiation, Cell Survival, Flow Cytometry, Fluorescent Antibody Technique, Mice, Mutation, Phospholipase C gamma, Proto-Oncogene Proteins c-bcl-2, Receptors, Antigen, B-Cell, Signal Transduction, Type C Phospholipases