Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The T cell repertoire is shaped by positive and negative selection of thymocytes. TCR-mediated signals that determine these selection processes are only partly understood. The CD45 tyrosine phosphatase has been shown to be important for signal transduction through the TCR, but there has been disagreement about whether CD45 is a positive or negative regulator of TCR signaling. Using CD45-deficient mice expressing transgenic TCR, we show that in the absence of CD45 there is a large increase in the thresholds of TCR stimulation required for both positive and negative selection. Our results conclusively demonstrate that in double-positive thymocytes CD45 is a positive regulator of the TCR signals that drive thymic selection events.

Original publication

DOI

10.1002/(SICI)1521-4141(199909)29:09<2923::AID-IMMU2923>3.0.CO;2-Y

Type

Journal article

Journal

Eur J Immunol

Publication Date

09/1999

Volume

29

Pages

2923 - 2933

Keywords

Animals, Calcium, Embryo, Mammalian, Female, Gene Targeting, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Leukocyte Common Antigens, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Mutagenesis, Site-Directed, Protein Tyrosine Phosphatases, Signal Transduction, Stem Cells, Thymus Gland