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ADP-ribosylation is the process of transferring the ADP-ribose moiety from NAD+ to a substrate. While a number of proteins represent well described substrates accepting ADP-ribose modification, a recent report demonstrated biological role for DNA ADP-ribosylation as well. The conserved macrodomain fold of several known hydrolyses was found to possess de-ADP-ribosylating activity and the ability to hydrolyze (reverse) ADP-ribosylation. Here we summarize the methods that can be employed to study mono-ADP-ribosylation hydrolysis by macrodomains.

Original publication

DOI

10.1007/978-1-4939-8588-3_14

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2018

Volume

1813

Pages

215 - 223

Keywords

ADP-ribosylation, Hydrolysis, Macrodomain fold, NAD+, Toxin-antitoxin system