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A large number of microclones obtained by microdissection of the mouse X chromosome have been mapped using an interspecific Mus domesticus/Mus spretus cross. Clones displaying close linkage to a number of loci of known phenotype but unknown gene product, such as mdx (X-linked muscular dystrophy), have been obtained. Over a central 30 cM span of the mouse X chromosome, 17 clones have been mapped and ordered at a sufficient density to contemplate the complete physical mapping of this region that will aid in the isolation of a number of unidentified genes. Some of the mapped microclones detect moderately repetitive sequences that were clustered in several discrete regions of the mouse X chromosome.


Journal article



Publication Date





3291 - 3297


Animals, Chromosome Mapping, Cloning, Molecular, Crosses, Genetic, DNA Restriction Enzymes, Deoxyribonuclease HpaII, Deoxyribonucleases, Type II Site-Specific, Female, Male, Mice, Muridae, Recombination, Genetic, X Chromosome