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Since 1992, the proportion of culture-confirmed meningococcal infections compared with numbers of notified cases of meningococcal disease has decreased in England and Wales. As most meningococcal strain characterization methods depend on a clinical isolate, this has resulted in a loss of epidemiological information. To address this problem, and to aid nonculture diagnosis, a semi-nested PCR protocol for the amplification of the meningococcal porB gene from clinical specimens was developed. This gene encodes the meningococcal serotyping antigen; strain typing data was provided by hybridization of allele-specific oligonucleotide probes to the digoxigenin-labelled porB amplicon in a 'PCR ELISA'. This assay was specific for meningococcal DNA and sensitivities of 0.81 for cerebrospinal fluid (CSF), 0.57 for serum, and 0.62 for whole blood taken from patients with proven meningococcal infection were obtained.

Type

Journal article

Journal

Epidemiol Infect

Publication Date

06/1998

Volume

120

Pages

257 - 262

Keywords

Bacterial Outer Membrane Proteins, DNA, Bacterial, Enzyme-Linked Immunosorbent Assay, Neisseria meningitidis, Oligonucleotide Probes, Polymerase Chain Reaction, Porins, Serotyping