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There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and color plane alignment using confocal and structured illumination fluorescence microscopes.

Original publication

DOI

10.1364/OE.26.021887

Type

Journal article

Journal

Opt Express

Publication Date

20/08/2018

Volume

26

Pages

21887 - 21899