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Among a series of adhesion molecules, expression of integrin alpha 4 beta 1 showed a unique inverse correlation with the invasive potential of B16 melanoma cell lines. When an alpha 4 cDNA was introduced into an alpha 4-beta 1+ highly invasive melanoma line, alpha 4 beta 1 heterodimers were expressed on the surface. Matrigel invasion by the alpha 4+ beta 1+ cells was reduced. Pulmonary metastasis was also suppressed when the transfectants were placed subcutaneously, but not when injected intravenously. Expression of alpha 4 beta 1 promoted homotypic intercellular adhesion. The homotypic adhesion was abrogated, and the alpha 4+ beta 1+ (less invasive cell lines) increased matrigel invasion following the anti-alpha 4 MAb treatment. These results suggest that integrin alpha 4 beta 1 could play a role in controlling melanoma cell metastasis at the invasive stage.

Type

Journal article

Journal

Cell

Publication Date

06/05/1994

Volume

77

Pages

335 - 347

Keywords

Animals, Antibodies, Monoclonal, Cell Adhesion, Cell Adhesion Molecules, Collagen, DNA, Complementary, Drug Combinations, Genetic Vectors, Integrin alpha4beta1, Integrins, Laminin, Lung Neoplasms, Male, Melanoma, Experimental, Mice, Mice, Inbred C57BL, Neoplasm Invasiveness, Proteoglycans, RNA, Messenger, Receptors, Very Late Antigen, Retroviridae, Tumor Cells, Cultured