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CpG islands are regions of vertebrate genomes that often function as gene regulatory elements and are associated with most gene promoters. CpG island elements usually contain nonmethylated CpG dinucleotides, while the remainder of the genome is pervasively methylated. We developed a biochemical approach called biotinylated CxxC affinity purification (BioCAP) to unbiasedly isolate regions of the genome that contain nonmethylated CpG dinucleotides. The resulting highly pure nonmethylated DNA is easily analyzed by quantitative PCR to interrogate specific loci or via massively parallel sequencing to yield genome-wide profiles.

Original publication

DOI

10.1007/978-1-4939-7768-0_2

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2018

Volume

1766

Pages

15 - 29

Keywords

Biotinylated CxxC affinity purification, CpG island, DNA methylation, Nonmethylated DNA, Nonmethylated island