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Gigaohm recordings have been made from glutamate receptor channels in excised, outside-out patches of collagenase-treated locust muscle membrane. The channels in the excised patches exhibit the kinetic state switching first seen in megaohm recordings from intact muscle fibers. Analysis of channel dwell time distributions reveals that the gating mechanism contains at least four open states and at least four closed states. Dwell time autocorrelation function analysis shows that there are at least three gateways linking the open states of the channel with the closed states. A maximum likelihood procedure has been used to fit six different gating models to the single channel data. Of these models, a cooperative model yields the best fit, and accurately predicts most features of the observed channel gating kinetics.

Original publication

DOI

10.1016/S0006-3495(90)82367-5

Type

Journal article

Journal

Biophys J

Publication Date

07/1990

Volume

58

Pages

219 - 229

Keywords

Animals, Electrophysiology, Glutamates, Glutamic Acid, Grasshoppers, In Vitro Techniques, Ion Channels, Kinetics, Mathematics, Models, Biological, Muscles, Receptors, Glutamate, Receptors, Neurotransmitter