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The conformations of the metal-bound 5'-ribonucleotides and 5'-deoxyribonucleotides in aqueous solution at different pH values have been studied using the lanthanide probe method. The conformational analysis, based on mixing different conformations in fast exchange within the nuclear magnetic resonance time scale, agrees well with the results from coupling constants, nuclear Over-hauser effects and spin-lattice relaxation times, obtained for the metal-fixed systems. The equilibrium between the two basic conformational combinations for the 5'-nucleotides, anti-(N in equalibrium S)-gg-g'g' and syn-(N in equalibrium S)-gt-g'g' depends on the nature of the furanose ring, the base and also on the state of base protonation and phosphate ionization. The effect of base protonation is particularly strong for the guanine nucleotides.

Type

Journal article

Journal

Eur J Biochem

Publication Date

17/07/1978

Volume

88

Pages

259 - 266

Keywords

Adenosine Monophosphate, Cytidine Monophosphate, Deoxyribonucleotides, Guanosine Monophosphate, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Metals, Rare Earth, Nucleic Acid Conformation, Ribonucleotides, Water