Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

There is a range of diseases of the brain that are associated with rapidly developing inflammation. These conditions are called encephalitis, and can be caused by infections, or autoimmune disorders when the subject's immune system reacts against host proteins. The most common form of autoimmune encephalitis is associated with antibodies against the NR1 subunit of the N-Methyl-D-Aspartate Receptor (anti-NMDAR-encephalitis). Antibody detection is important for diagnosis, and can be performed only with techniques that preserve the full tertiary structure (conformation) of the antigen. Here, we describe the methods commonly used to detect NMDAR-antibodies with immunohistochemistry on rat brain slices and on live HEK cells transfected with components of the NMDAR.

Original publication




Journal article


Methods Mol Biol

Publication Date





117 - 126


Autoantibody, Encephalitis, NMDAR, Animals, Anti-N-Methyl-D-Aspartate Receptor Encephalitis, Autoantibodies, Brain, Cell Line, Encephalitis, Humans, Immunohistochemistry, Rats, Receptors, N-Methyl-D-Aspartate