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When plants are infected with pathogens they defend themselves via various processes. Once such process is the development of the hypersensitive response (HR), a kind of programmed cell death (PCD), in which localized cell death takes place in order to prevent pathogen spread to other part of tissue. The Arabidopsis and Pseudomonas syringae system is one of the best known examples to study the HR. Here we used the VisiSens™ oxygen-imaging system to investigate oxygen distributions in Arabidopsis leaves infected with an avirulent strain of P. syringae and undergoing the hypersensitive response. Using this method we observed a change in oxygen status at 6 h post-infection and a drop in oxygen levels at 24 h after infection.

Original publication




Journal article


Methods Mol Biol

Publication Date





71 - 76


Arabidopsis, Hypersensitive response, Oxygen, Programmed cell death, VisiSens, Arabidopsis, Calibration, Imaging, Three-Dimensional, Oxygen, Plant Diseases, Plant Leaves, Pseudomonas syringae