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Whilst photorespiration represents one of the dominant pathway fluxes in photosynthetic tissues there are hints from publically available gene expression data such as that housed in the bioarray resource (BAR; www.bar.utoronto.ca) that several of the constituent enzymes are present in roots and other heterotrophic tissues. Here we describe a protocol based on modification of the gaseous environment surrounding individual tissues of mutant and wild type Arabidopsis and evaluation of the consequences. This method could additionally easily be used for larger plants.

Original publication

DOI

10.1007/978-1-4939-7225-8_15

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2017

Volume

1653

Pages

217 - 225

Keywords

Metabolite analysis, Photorespiratory mutants, Split-plant gas experiment, Arabidopsis, Carbon Dioxide, Gas Chromatography-Mass Spectrometry, Hydroponics, Metabolic Networks and Pathways, Metabolome, Mutation, Oxygen, Oxygen Consumption, Photosynthesis, Plant Roots, Seedlings, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization