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By combining the capabilities of advanced sample preparation methodologies with the latest generation of secondary ion mass spectrometry instrumentation, we show that chemical information on the distribution of even dilute species in biological samples can be obtained with spatial resolutions of better than 100 nm. Here, we show the distribution of nickel and other elements in leaf tissue of the nickel hyperaccumulator plant Alyssum lesbiacum prepared by high-pressure freezing and freeze substitution.

Original publication

DOI

10.1111/j.1365-313X.2010.04279.x

Type

Journal article

Journal

Plant J

Publication Date

09/2010

Volume

63

Pages

870 - 879

Keywords

Brassicaceae, Microscopy, Electron, Transmission, Nanotechnology, Nickel, Plant Leaves, Spectrometry, Mass, Secondary Ion, Spectrophotometry, Atomic, Vacuoles