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Rhizobium leguminosarum has two high-affinity Mn(2+) transport systems encoded by sitABCD and mntH. In symbiosis, sitABCD and mntH were expressed throughout nodules and also strongly induced in Mn(2+) -limited cultures of free-living cells. Growth of a sitA mntH double mutant was severely reduced under Mn(2+) limitation and sitA and mntH single mutants were more sensitive to oxidative stress. The double sitA mntH mutant of R. leguminosarum was unable to fix nitrogen (Fix(-) ) with legumes belonging to the galegoid clade (Pisum sativum, Vicia faba and Vicia hirsuta). The presence of infection thread-like structures and sparsely-packed plant cells in nodules suggest that bacteroid development was blocked, either at a late stage of infection thread progression or during bacteroid-release. In contrast, a double sitA mntH mutant was Fix(+) on common bean (Phaseoli vulgaris), a member of the phaseoloid clade of legumes, indicating a host-specific symbiotic requirement for Mn(2+) transport.

Original publication

DOI

10.1111/1462-2920.13773

Type

Journal article

Journal

Environ Microbiol

Publication Date

07/2017

Volume

19

Pages

2715 - 2726