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This paper addresses the biocompatibility of fluids and surfactants in the context of microfluidics and more specifically in a drops-in-drops system for mammalian cell based drug screening. In the drops-in-drops approach, three immiscible fluids are used to manipulate the flow of aqueous microliter-sized drops; it enables merging of drops containing cells with drops containing drugs within a Teflon tube. Preliminary tests showed that a commonly-used fluid and surfactant combination resulted in significant variability in gene expression levels in Jurkat cells after exposure to a drug for four hours. This result led to further investigations of potential fluid and surfactant combinations that can be used in microfluidic systems for medium to long-term drug screening. Results herein identify a fluid combination, HFE-7500 and 5-cSt silicone oil + 0.25% Abil EM180, which enabled the drops-in-drops approach; this combination also allowed gene expression at normal levels comparable with the conventional drug screening in both magnitude and variability.

Original publication

DOI

10.1007/s10544-016-0137-0

Type

Journal article

Journal

Biomed Microdevices

Publication Date

12/2016

Volume

18

Keywords

Biocompatibility, Drops-in-drops, Drug screening, Mammalian cell, Surfactant, Toxicity, Animals, Cell Survival, Drug Evaluation, Preclinical, Humans, Jurkat Cells, Lab-On-A-Chip Devices, Materials Testing, Mice, Surface-Active Agents