In Vitro-Pooled shRNA Screening to Identify Determinants of Radiosensitivity.
Ceroni A., Higgins GS., Ebner DV.
Short hairpin RNA (shRNA)-pooled screening is a valuable and cost-effective tool for assaying the contribution of individual genes to cell viability and proliferation on a genomic scale. Here we describe the key considerations for the design and execution of a pooled shRNA screen to identify determinants of radiosensitivity.