Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Cohesin is a multi-subunit protein complex essential for sister chromatid cohesion, gene expression and DNA damage repair. Although structurally well studied, the underlying determinant of cohesion establishment on chromosomal arms remains enigmatic. Here, we show two populations of functionally distinct cohesin on chromosomal arms using a combination of genomics and single-locus specific DNA-FISH analysis. Chromatin bound cohesin at the loading sites co-localizes with Pds5 and Eso1 resulting in stable cohesion. In contrast, cohesin independent of its loader is unable to maintain cohesion and associates with chromatin in a dynamic manner. Cohesive sites coincide with highly expressed genes and transcription inhibition leads to destabilization of cohesin on chromatin. Furthermore, induction of transcription results in de novo recruitment of cohesive cohesin. Our data suggest that transcription facilitates cohesin loading onto chromosomal arms and is a key determinant of cohesive sites in fission yeast.

Original publication

DOI

10.1093/nar/gkw252

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

19/08/2016

Volume

44

Pages

6676 - 6692

Keywords

Acetyltransferases, Cell Cycle Proteins, Chromatids, Chromatin, Chromatin Immunoprecipitation, Chromosomal Proteins, Non-Histone, Chromosomes, Fungal, DNA-Directed RNA Polymerases, Genes, Fungal, Genetic Loci, HEK293 Cells, Heat-Shock Response, Humans, In Situ Hybridization, Fluorescence, Protein Subunits, Saccharomyces cerevisiae, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Transcription, Genetic