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© Springer Science+Business Media New York 2015. All right reserved. Mitochondrial physiology sets the basis for function of the organelle and vice versa. While a limited range of in vivo parameters, such as oxygen consumption, has been classically accessible for measurement, a growing collection of fluorescent protein sensors can now give insights into the physiology of plant mitochondria. Nevertheless, the meaningful application of these sensors in mitochondria is technically challenging and requires rigorous experimental standards. Here we exemplify the application of three genetically encoded sensors to monitor glutathione redox potential, pH, and calcium in the matrix of mitochondria in intact plants. We describe current methods for quantitative imaging and analysis in living root tips by confocal microscopy and discuss methodological limitations.

Original publication

DOI

10.1007/978-1-4939-2639-8_17

Type

Chapter

Book title

Plant Mitochondria: Methods and Protocols

Publication Date

24/04/2015

Pages

241 - 252