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Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.

Original publication

DOI

10.1038/nmeth.3771

Type

Journal article

Journal

Nat Methods

Publication Date

04/2016

Volume

13

Pages

333 - 336

Keywords

Humans, Lipids, Mass Spectrometry, Membrane Proteins, Models, Molecular, Peptide Fragments, Protein Binding, Small Molecule Libraries