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Kinetochores are macromolecular machines that drive eukaryotic chromosome segregation by interacting with centromeric DNA and spindle microtubules. While most eukaryotes possess conventional kinetochore proteins, evolutionarily distant kinetoplastid species have unconventional kinetochore proteins, composed of at least 19 proteins (KKT1-19). Polo-like kinase (PLK) is not a structural kinetochore component in either system. Here, we report the identification of an additional kinetochore protein, KKT20, in Trypanosoma brucei. KKT20 has sequence similarity with KKT2 and KKT3 in the Cys-rich region, and all three proteins have weak but significant similarity to the polo box domain (PBD) of PLK. These divergent PBDs of KKT2 and KKT20 are sufficient for kinetochore localization in vivo. We propose that the ancestral PLK acquired a Cys-rich region and then underwent gene duplication events to give rise to three structural kinetochore proteins in kinetoplastids.

Original publication

DOI

10.1098/rsob.150206

Type

Journal article

Journal

Open Biol

Publication Date

03/2016

Volume

6

Keywords

DPB, Trypanosoma brucei, kinetochore, kinetoplastid, polo box domain, polo-like kinase, Amino Acid Sequence, Cell Cycle Proteins, Humans, Kinetochores, Mitosis, Protein Domains, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Protozoan Proteins, Sequence Alignment, Trypanosoma brucei brucei, Trypanosomiasis, African