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The nucleotide sequence is reported for a cDNA containing the entire coding region of a stearoyl-ACP desaturase (EC 1.14.99.6) from Brassica napus L. cv. Jet neuf. The cDNA was obtained from a library constructed from poly(A)+ RNA purified from embryo tissue. The derived amino acid sequence demonstrates substantial similarity with those from other plant delta 9-desaturases. Comparative RNA-dot blot analyses using the delta 9-desaturase cDNA and a rapeseed oleosin cDNA as probes showed that although both these transcripts were seed-specific, they exhibited distinct patterns of temporal regulation. The desaturase message was induced by 25 days after anthesis (DAA), peaking at 45 DAA but decreasing considerably thereafter. In contrast, the oleosin transcript did not increase until 45-50 DAA, reaching a peak much later at about 70 DAA.

Type

Journal article

Journal

Plant Mol Biol

Publication Date

10/1992

Volume

20

Pages

151 - 155

Keywords

Amino Acid Sequence, Base Sequence, Brassica, Cloning, Molecular, DNA, Gene Expression Regulation, Enzymologic, Mixed Function Oxygenases, Molecular Sequence Data, Sequence Homology, Nucleic Acid