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The gene-for-gene model postulates that for every gene determining resistance in the host plant, there is a corresponding gene conditioning avirulence in the pathogen. On the basis of this relationship, products of resistance (R) genes and matching avirulence (Avr) genes are predicted to interact. Here, we report on binding studies between the R gene product Cf-9 of tomato and the Avr gene product AVR9 of the pathogenic fungus Cladosporium fulvum. Because a high-affinity binding site (HABS) for AVR9 is present in tomato lines, with or without the Cf-9 resistance gene, as well as in other solanaceous plants, the Cf-9 protein was produced in COS and insect cells in order to perform binding studies in the absence of the HABS. Binding studies with radio-labeled AVR9 were performed with Cf-9-producing COS and insect cells and with membrane preparations of such cells. Furthermore, the Cf-9 gene was introduced in tobacco, which is known to be able to produce a functional Cf-9 protein. Binding of AVR9 to Cf-9 protein produced in tobacco was studied employing surface plasmon resonance and surface-enhanced laser desorption and ionization. Specific binding between Cf-9 and AVR9 was not detected with any of the procedures. The implications of this observation are discussed.

Original publication

DOI

10.1094/MPMI.2001.14.7.867

Type

Journal article

Journal

Mol Plant Microbe Interact

Publication Date

07/2001

Volume

14

Pages

867 - 876

Keywords

Animals, COS Cells, Cell Line, Cladosporium, Fungal Proteins, Genes, Fungal, Genes, Plant, Lycopersicon esculentum, Membrane Glycoproteins, Models, Genetic, Plant Diseases, Plant Proteins, Plants, Genetically Modified, Protein Binding, Recombinant Proteins, Spodoptera, Surface Plasmon Resonance, Tobacco, Virulence