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A 2-kb region that complements the Tn5-derived lipopolysaccharide (LPS) rough mutant Rhizobium leguminosarum RU301 was sequenced. Two open reading frames (ORFs) were identified. The first ORF (lpcA) is homologous to a family of bacterial sugar transferases involved in LPS core tetrasaccharide biosynthesis. ORF2 (lpcB), in which Tn5 transposed, has no significant homology to any DNA in the GenBank-EMBL databases. Chemical characterization of LPS produced by strain RU301 demonstrated that the 3-deoxy-D-manno-2-octulosonic acid (Kdo) residue which normally attaches the core tetrasaccharide to the O chain was missing, suggesting that IpcB may encode a CMP-Kdo:LPS Kdo transferase.

Type

Journal article

Journal

J Bacteriol

Publication Date

11/1996

Volume

178

Pages

6403 - 6406

Keywords

Amino Acid Sequence, Bacterial Proteins, Base Sequence, DNA, Bacterial, Hexosyltransferases, Lipopolysaccharides, Molecular Sequence Data, Mutation, Rhizobium leguminosarum, Sequence Homology, Amino Acid, Transferases