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ATP-binding cassette transporters from several rhizobia and Salmonella enterica serovar Typhimurium, but not secondarily coupled systems, were inhibited by high concentrations (100 to 500 mM) of various osmolytes, an effect reversed by the removal of the osmolyte. ABC systems were also inactivated in isolated pea bacteroids, probably due to the obligatory use of high-osmolarity isolation media. Measurement of nutrient cycling in isolated pea bacteroids is impeded by this effect.

Original publication

DOI

10.1128/JB.00262-06

Type

Journal article

Journal

J Bacteriol

Publication Date

07/2006

Volume

188

Pages

5304 - 5307

Keywords

ATP-Binding Cassette Transporters, Aminoisobutyric Acids, Biological Transport, Gram-Positive Bacteria, Kinetics, Salmonella typhimurium