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We characterized the time course of inflammatory cytokine release at the site of injury and in plasma after surgery on the rat tail. Anesthetized Sprague-Dawley rats had a 20 mm long incision made through the skin and fascia of their tails. Control rats were anesthetized, but no incision was made. Blood and tissue samples were taken 2 h and 1, 2, 4, and 8 days after surgery and analysed by ELISA for interleukin-1beta (IL-1beta), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and cytokine-induced neutrophil chemoattractant-1 (CINC-1). In another group of rats, daily behavioral measurements were made of the rats' responses to a blunt noxious mechanical stimulus (4 Newtons) applied to their tails. Primary hyperalgesia developed within 2 h of surgery and lasted for 6 days. The tissue concentrations of IL-1beta, IL-6, and CINC-1 increased within 24 h of surgery, and TNF-alpha concentration increased within 48 h of surgery. Thereafter, cytokine concentrations remained elevated for 4 (IL-1beta and IL-6) to 8 days (CINC-1, TNF-alpha) after surgery. Control animals did not develop hyperalgesia and no changes in cytokines concentrations were detected. Thus, in our model of postoperative pain, secretion of inflammatory cytokines IL-1beta, IL-6, TNF-alpha, and CINC-1 was not essential for the initiation of postoperative hyperalgesia.

Original publication

DOI

10.1139/y07-054

Type

Journal article

Journal

Can J Physiol Pharmacol

Publication Date

06/2007

Volume

85

Pages

613 - 620

Keywords

Animals, Chemokine CXCL1, Chemokines, CXC, Cytokines, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Female, Hyperalgesia, Inflammation, Inflammation Mediators, Interleukin-1beta, Interleukin-6, Male, Pain, Postoperative, Rats, Rats, Sprague-Dawley, Tail, Time Factors, Tumor Necrosis Factor-alpha, Up-Regulation