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AtCyp59 and its orthologs from different organisms belong to a family of modular proteins consisting of a peptidyl-prolyl cis-trans isomerase (PPIase) domain, followed by an RNA recognition motif (RRM), and a C-terminal domain enriched in charged amino acids. AtCyp59 was identified in a yeast two-hybrid screen as an interacting partner of the Arabidopsis SR protein SCL33/SR33. The interaction with SCL33/SR33 and with a majority of Arabidopsis SR proteins was confirmed by in vitro pull-down assays. Consistent with these interactions, AtCyp59 localizes to the cell nucleus, but it does not significantly colocalize with SR proteins in nuclear speckles. Rather, it shows a punctuate localization pattern resembling transcription sites. Indeed, by using yeast two-hybrid, in vitro pull-down, and immunoprecipitation assays, we found that AtCyp59 interacts with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II. Ectopic expression of the tagged protein in Arabidopsis cell suspension resulted in highly reduced growth that is most probably due to reduced phosphorylation of the CTD. Together our data suggest a possible function of AtCyp59 in activities connecting transcription and pre-mRNA processing. We discuss our data in the context of a dynamic interplay between transcription and pre-mRNA processing.

Original publication

DOI

10.1261/rna.2226106

Type

Journal article

Journal

RNA

Publication Date

04/2006

Volume

12

Pages

631 - 643

Keywords

Amino Acid Sequence, Arabidopsis Proteins, Base Sequence, Binding Sites, Blotting, Western, Cell Nucleus, Cloning, Molecular, Cyclophilins, DNA Primers, DNA, Complementary, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Phosphorylation, RNA Polymerase II, RNA, Plant, Sequence Homology, Amino Acid, Two-Hybrid System Techniques