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Land plants develop filamentous cells-root hairs, rhizoids, and caulonemata-at the interface with the soil. Members of the group XI basic helix-loop-helix (bHLH) transcription factors encoded by LOTUS JAPONICUS ROOTHAIRLESS1-LIKE (LRL) genes positively regulate the development of root hairs in the angiosperms Lotus japonicus, Arabidopsis thaliana, and rice (Oryza sativa). Here we show that auxin promotes rhizoid and caulonema development by positively regulating the expression of PpLRL1 and PpLRL2, the two LRL genes in the Physcomitrella patens genome. Although the group VIII bHLH proteins, AtROOT HAIR DEFECTIVE6 and AtROOT HAIR DEFECTIVE SIX-LIKE1, promote root-hair development by positively regulating the expression of AtLRL3 in A. thaliana, LRL genes promote rhizoid development independently of PpROOT HAIR DEFECTIVE SIX-LIKE1 and PpROOT HAIR DEFECITVE SIX-LIKE2 (PpRSL1 and PpRSL2) gene function in P. patens. Together, these data demonstrate that both LRL and RSL genes are components of an ancient auxin-regulated gene network that controls the development of tip-growing cells with rooting functions among most extant land plants. Although this network has diverged in the moss and the angiosperm lineages, our data demonstrate that the core network acted in the last common ancestor of the mosses and angiosperms that existed sometime before 420 million years ago.

Original publication

DOI

10.1073/pnas.1416324112

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

21/07/2015

Volume

112

Pages

E3959 - E3968

Keywords

auxin, bHLH, evolution, rhizoids, root hairs, Amino Acid Sequence, Basic Helix-Loop-Helix Transcription Factors, Bayes Theorem, Bryopsida, Conserved Sequence, Gene Expression Regulation, Plant, Genes, Plant, Glucuronidase, Indoleacetic Acids, Likelihood Functions, Models, Biological, Molecular Sequence Data, Mutation, Phenotype, Phosphates, Phylogeny, Plant Proteins, Plant Roots, Sequence Alignment