Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

In addition to inter-chromatid cohesion, mitotic and meiotic chromatids must have three physical properties: compaction into 'threads' roughly co-linear with their DNA sequence, intra-chromatid cohesion determining their rigidity, and a mechanism to promote sister chromatid disentanglement. A fundamental issue in chromosome biology is whether a single molecular process accounts for all three features. There is universal agreement that a pair of Smc-kleisin complexes called condensin I and II facilitate sister chromatid disentanglement, but whether they also confer thread formation or longitudinal rigidity is either controversial or has never been directly addressed respectively. We show here that condensin II (beta-kleisin) has an essential role in all three processes during meiosis I in mouse oocytes and that its function overlaps with that of condensin I (gamma-kleisin), which is otherwise redundant. Pre-assembled meiotic bivalents unravel when condensin is inactivated by TEV cleavage, proving that it actually holds chromatin fibres together.

Original publication

DOI

10.1038/ncb3167

Type

Journal article

Journal

Nat Cell Biol

Publication Date

06/2015

Volume

17

Pages

771 - 781

Keywords

Adenosine Triphosphatases, Animals, Cell Cycle Proteins, Chromatids, Chromosomal Proteins, Non-Histone, Chromosome Segregation, Chromosomes, DNA-Binding Proteins, Meiosis, Mice, Mice, Transgenic, Multiprotein Complexes, Nuclear Proteins, Oocytes