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OxF1 is a human embryonic stem cell line derived from a surplus embryo donated through the Oxford IVF clinic. The cells have a stable 46 XX karyotype and show expression of Oct 4, Nanog and TRA-1-60. Embryoid bodies differentiate into cells that represent all three germ layers as demonstrated by immunohistochemical localisation of beta III tubulin, nestin, desmin, smooth muscle actin, Gata 6 and cytokeratin 18. Directed differentiation through haematopoiesis has been demonstrated.

Original publication

DOI

10.1007/s11626-010-9283-5

Type

Journal article

Journal

In Vitro Cell Dev Biol Anim

Publication Date

04/2010

Volume

46

Pages

173 - 177

Keywords

Cell Culture Techniques, Cell Differentiation, Cell Line, Cell Lineage, Embryo, Mammalian, Embryonic Stem Cells, Germ Layers, Humans, Myeloid Cells, United Kingdom