Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

A rabbit polyclonal antiserum was raised against a synthetic peptide, termed CD2-300, comprizing 18 amino acid resides which are conserved among the cytoplasmic domains of the human, rat and mouse CD2 antigens. Cross-depletion experiments showed that the CD2 monoclonal antibody OKT11 and purified CD2-300 antibodies (Ab) precipitated the same molecules from the surface of human T lymphoblasts. The results of immunoprecipitation analyses indicated that the purified CD2-300 Ab were specific for human and mouse CD2, and that the CD2-300 peptide competitively and specifically inhibited precipitation by CD2-300 Ab in both species. When employed to stain murine tissues, the CD2-300 Ab gave the anticipated pattern of distribution for the CD2 antigen, although there was some nonspecific labeling of non-T cells.

Original publication




Journal article


Eur J Immunol

Publication Date





1223 - 1227


Animals, Antigens, Differentiation, CD2 Antigens, Chemical Precipitation, Cross Reactions, Humans, Immunoenzyme Techniques, Mice, Oligopeptides, Palatine Tonsil, Receptors, Immunologic, T-Lymphocytes