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Endolysosomes are emerging as key players that generate as well as respond to intracellular Ca(2+) signals. The role of Ca(2+) in modulating acidic organelle function has long been recognized, but it is now emerging that acidic organelles also act as intracellular Ca(2+) stores; they actively sequester Ca(2+) in their lumina and release it to the cytosol upon activation of endolysosomal Ca(2+) channels. This local Ca(2+) signal is crucial for endolysosomal function and/or global Ca(2+) signaling. Importantly, defects in endolysosomal Ca(2+) are associated with disease. This chapter discusses several complimentary approaches to monitor endolysosomal Ca(2+), with particular emphasis on the inherent pitfalls that can plague the unwary.

Original publication




Journal article


Methods Cell Biol

Publication Date





159 - 195


Bafilomycin A1, Ca(2+), GPN, Lysosome, NAADP, Nigericin, pH, Animals, Calcium, Calcium Signaling, Cells, Cultured, Endosomes, Fluorescent Dyes, Homeostasis, Humans, Hydrogen-Ion Concentration, Lysosomes, Microscopy, Fluorescence, Staining and Labeling