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Surface-coat epitopes of Meloidogyne incognita were detected in root tissues of Arabidopsis thaliana during migration and feeding site formation. A whole-mount root technique was used for immunolocalization of surface coat epitopes in A. thaliana, with the aid of a monoclonal antibody raised specifically against the outer surface of infective juveniles of M. incognita. The antibody, which was Meloidogyne-specific, recognized a fucosyl-bearing glycoprotein in the surface coat. During migration in host tissues the surface coat was shed, initially accumulating in the intercellular spaces next to the juvenile and later at cell junctions farther from the nematode. Upon induction of giant cell formation, the antibody bound to proximally located companion cells and sieve elements of the phloem.

Type

Journal article

Journal

J Nematol

Publication Date

06/1999

Volume

31

Pages

212 - 223

Keywords

Arabidopsis thaliana, Meloidogyne incognita, antigen, antigenic mimicry, cell wall, host-parasite relationship, immunolocalization, migration, nematode, surface coat, ultrastructure, whole-mount root technique