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Ypt/Rab GTPases act as key regulators of intracellular traffic through the conformational differences exhibited by their GTP or GDP-bound forms. In this paper, two Arabidopsis Ypt6 homologues, AtRAB-H1(b) and AtRAB-H1(c) were characterized and compared. Using a live cell imaging approach, it is shown that yellow fluorescent protein-fusions (YFP) of AtRAB-H1(b) and AtRAB-H1(c) locate to the Golgi and to the cytosol in both Nicotiana tabacum and in Arabidopsis thaliana. In addition, YFP-AtRAB-H1(b) targets an as yet unknown compartment not labelled by YFP-AtRAB-H1(c) or Golgi markers. It is also shown that the subcellular location of YFP-AtRAB-H1(b) and YFP-AtRAB-H1(c) is affected by the state of GTP-binding and that expression of a GTP-deficient mutant results in increased apoplastic fluorescence of a secretory form of YFP.

Original publication

DOI

10.1093/jxb/erp153

Type

Journal article

Journal

J Exp Bot

Publication Date

2009

Volume

60

Pages

3179 - 3193

Keywords

Arabidopsis, Arabidopsis Proteins, Cytosol, Gene Expression, Genes, Reporter, Golgi Apparatus, Guanosine Triphosphate, Protein Binding, Protein Transport, Recombinant Fusion Proteins, Tobacco, rab GTP-Binding Proteins